Composite

Part:BBa_K2920001:Design

Designed by: Chung Hui Hsu   Group: iGEM19_Tunghai_TAPG   (2019-09-01)


Peptide production process


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 362
    Illegal NheI site found at 1904
    Illegal NheI site found at 2664
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1570
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2447
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005


Design Notes

3.4. Procedure

1. Identify target gene

2. Design primer

3. Polymerase chain reaction

4. DNA purification

5. Extract target DNA from plasmid

6. First transformation

7. Confirm fragment size of plasmid

8. Sequencing

9. Second transformation

10. Protein expression

11. Identify purification conditions

12. Protein expression and purification

13. Antimicrobial activity analysis



Source

tunghai university and kit

References